Figure 1
From: Inhibition of Monkeypox virus replication by RNA interference
Representative results from screened siRNA pools designed to specifically target selected MPV genes. MK2 cells were transfected with a set of four multiplexed siRNA constructs targeting a single gene at a time. Parallel control cells were transfected with scrambled siRNAs under identical conditions to ensure antiviral specificity. Preliminary siRNA pools evaluation was carried at concentrations of 100 and 200 nM. Transfected cells were infected with MPV, and viral replication was assayed after incubation for 48 h by plaque assay. Presented results are relative to viral replication in mock-transfected cells ± one standard deviation. Multiplexed siRNA pools targeting A6R and E8L genes, i.e., siA6 and siE8, exhibited a strong dose-dependent antiviral effect with no or negligible toxicity at both concentrations.