HTS screening of a small compound library against live NiV. Cells were treated with 10 μM of each compound (100 μl) immediately prior to infection with 1,000 TCID50 NiV in 100 μl. Cells were incubated overnight at 37°C. Monolayers were fixed with methanol, air dried and immunostained with anti-NiV-N polyclonal antisera as described above. (a) Dose response curves were generated for 28 compounds with >90% inhibition (10 μM) showing a range of 50% inhibitory concentration (IC50) values all less than 2 μM (n = 3). (b) A wide range of 50% cytotoxicity concentration (CC50) values was observed for these 28 compounds (n = 3). Values determined for brilliant green (BG), gentian violet (GV) and gliotoxin (glio) are indicated.