Figure 2From: Bovine viral diarrhea virus NS4B protein is an integral membrane protein associated with Golgi markers and rearranged host membranesKinetics of BVDV NS3 protein expression in infected cells. MDBK cells were infected with BVDV at an MOI of 5 and the cell lysates prepared at the indicated time points. Rabbit anti-NS3 polyclonal antibody was used at 1/1000 dilution. Goat anti-rabbit alkaline phosphatase-conjugated secondary antibody was used to detect NS3 protein. M: Mock-infected cell lysate collected at 24 h after incubation. Higher NS3 expression levels are seen at 24 h and 48 h.p.i.Back to article page