Figure 11From: Bovine viral diarrhea virus NS4B protein is an integral membrane protein associated with Golgi markers and rearranged host membranesColocalization of BVDV NS4B with replicase proteins NS5A and NS5B. BHK-21 cells were co-transfected with NS4B-GFP and NS5A-His or NS5B-HA. At 48 h p.t., the cells were processed for IF with either anti-His or anti-HA antibody (dilution 1/50). NS4B was detected via GFP fluorescence. Colocalization of NS4B-GFP (green) and NS5AHis (red) or NS5B-HA (red) results in yellow color. Bars = 10 μm.Back to article page