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Figure 10 | Virology Journal

Figure 10

From: Bovine viral diarrhea virus NS4B protein is an integral membrane protein associated with Golgi markers and rearranged host membranes

Figure 10

Subcellular distribution of BVDV NS4B in transfected cells. BHK-21 cells were transfected with NS4B-GFP. At 48 h p.t., the cells were processed for fluorescence microscopy. ER-Tracker (i-iii; a), LysoTracker (x-xii; d) and MitoTracker (xiii-xv; e) were used as markers for the ER, lysosome and mitochondria, respectively. Golgin-97 (iv-vi; b) and Rab5 (vii-ix; c) were used as markers for the Golgi apparatus and early endosome, respectively. NS4B was detected via GFP fluorescence. Colocalization of NS4B (green) with the cognate intracellular marker (red) results in yellow color. Notice the Colocalization of NS4B with Golgin-97 (b) and MitoTracker (e). Bars = 10 μm.

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Virology Journal

ISSN: 1743-422X