Figure 4

500 stable transductions of 2.2, 3.2, 4.3, 5.3 and 6.3. The results from both PCR and dot blot assays were summarized into 3 panel plots for each set of populations, with individual cassettes shown as dots in the top two panels (not detected and detected cassettes respectively), and the combination length measured by electrophoresis in the bottom panel. Some samples, mostly for 3.2 and 4.3, were excluded from analysis because there were either no colonies recovered from selection, or the neo^r control PCR was negative (green dots). Samples with disparate results between the two methods are indicated by red dots. The data shown is representative of 2 independently repeated amplification and detection experiments.