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Table 1 Number of copies of the DWV genome in tested bees using real-time RT-PCR.

From: Localization of deformed wing virus (DWV) in the brains of the honeybee, Apis mellifera Linnaeus

Bee Number Hive Date Sampled Symptomatic DWV copies/μg total RNA
1 B July 2007 - <103
2 D July 2007 - 1 × 105
3 D July 2007 - 5 × 106
4 D Sept. 2007 - 6 × 106
5 D Sept. 2007 + 4 × 107
6 D Sept. 2007 + 5 × 107
  1. The number of copies of viral RNA per μg of total bee RNA was calculated for the honeybees shown in Figures 1 and 2 using real-time RT-PCR. The copy number was calculated from a standard curve of a plasmid containing the DWV amplicon. Actin was used as an internal control and the Ct numbers were within 2.0 from each other.
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