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Table 1 Number of copies of the DWV genome in tested bees using real-time RT-PCR.

From: Localization of deformed wing virus (DWV) in the brains of the honeybee, Apis mellifera Linnaeus

Bee Number

Hive

Date Sampled

Symptomatic

DWV copies/μg total RNA

1

B

July 2007

-

<103

2

D

July 2007

-

1 × 105

3

D

July 2007

-

5 × 106

4

D

Sept. 2007

-

6 × 106

5

D

Sept. 2007

+

4 × 107

6

D

Sept. 2007

+

5 × 107

  1. The number of copies of viral RNA per μg of total bee RNA was calculated for the honeybees shown in Figures 1 and 2 using real-time RT-PCR. The copy number was calculated from a standard curve of a plasmid containing the DWV amplicon. Actin was used as an internal control and the Ct numbers were within 2.0 from each other.
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Virology Journal

ISSN: 1743-422X