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Figure 3 | Virology Journal

Figure 3

From: A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus

Figure 3

Validation of pAdhsp70E1Δ55k. a. Schema of the recombinant Ad plasmid pAdhsp70E1Δ55k. b. Restriction analysis to confirm the structure of pAdhsp70E1Δ55k by XbaI/KpnI E1 cleavage. The diagnostic fragment is marked with an arrow. Control was the Adeno-X Ad backbone plasmid. c. Standard PCR was used to confirm the correct structure of the recombinant CRAd-Adhsp70E1Δ55k. The purified recombinant virus was directly applied in the various indicated PCR reactions encompassing the modified E1 gene within the PI-SceI/I-CeuI site of the recombinant CRAd.

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