Figure 2From: A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirusValidation of hsp70E1Δ55k fragment. a. Schema of the recombinant hsp70E1Δ55k cassette obtained by XhoI/KpnI digestion of phsp70E1. b. The construct of phsp70E1 (see Fig. 1c) was confirmed by PCR using complementary oligonucleotide amplimers of sequences flanking the indicated fragments of the template plasmid. c. Candidate clones for phsp70E1Δ55k (see Fig. 1e) were screened using digestion analysis with KpnI and XbaI. Diagnostic fragments obtained are marked with an arrow. M-The 1 kb DNA ladder from Gibco was used as DNA size marker.Back to article page