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Figure 6 | Virology Journal

Figure 6

From: Comparative functional analysis of Jembrana disease virus Tat protein on lentivirus long terminal repeat promoters: evidence for flexibility at its N-terminus

Figure 6

Flexible properties of the jTat N terminus. (A) Schematic representation of fusion proteins tagged at the jTat N- or C-terminus. (B) LTR activation in permissive cells by wild-type jTat (set to 100%) or jTat with a fusion at either terminus. Dashed lines mark 20% (lower line) and 60% (upper line). (C) Comparison of hCycT1 or bovine bCycT1 affinity between jTat with N- or C-terminal fusions. BD-J; Gal4 BD at the jTat N terminus. (D) HIV, BIV and JDV LTR activites stimulated by two chimeric Tat proteins, jN17-bTat and jN21-hTat(upper panel), and wild-type jTat (set to 100%). Boxed residues represent wild-type jTat. (E) Possible structures of the Tat/CycT1/TAR ternary complexes. TAR RNA secondary structure includes stalk, bulge and loop. The compositions of the ternary complexes are listed below.

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Virology Journal

ISSN: 1743-422X