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Figure 5 | Virology Journal

Figure 5

From: Comparative functional analysis of Jembrana disease virus Tat protein on lentivirus long terminal repeat promoters: evidence for flexibility at its N-terminus

Figure 5

jTat residues essential for the interaction with CycT1. (A) HeLa cells were cotransfected with BD-bT1, the indicated Tat-NFκB fusion plasmid (detailed in Fig. 4B), and pFR-luc. Induced activation of pFR-luc reporter by wild-type jTat is set to 100%. Percent induction by jTat mutants is shown. The interaction partner utilized in this experiment is bCycT1. (B) The role of jTat N-terminal residues in CycT1 binding. Binding affinities are measured between jTat N-terminal truncation mutants and different CycT1 species.

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Virology Journal

ISSN: 1743-422X