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Figure 2 | Virology Journal

Figure 2

From: Comparative functional analysis of Jembrana disease virus Tat protein on lentivirus long terminal repeat promoters: evidence for flexibility at its N-terminus

Figure 2

jTat RBD residues critical for function. (A) Schematic representation of the sequence near the RBD of hTat and jTat (upper) and chimeric proteins bearing hTat AD and jTat RBD (lower). Shaded characters indicate residues evaluated by deletion analysis. hTat AD; hTat residues 1-47. (B) Percentages of LTR activation by different chimeric Tat proteins are shown and those by HJ66 are set to 100%. (C) Sequence alignment among jTat, bTat and three mutants. Boxed bold characters indicate residues differing from wild-type jTat. (D) Activities of mutant and wild-type jTat proteins, bTat and hTat on the HIV LTR reporter in HeLa cells. jTat activity is set to 100%. (E) Schematic representation of chimeric proteins bearing either bTat AD and jTat RBD (BJ) or jTat AD and bTat RBD (JB). (F) Activities of mutant jTat proteins, chimeric proteins, and wild-type bTat and jTat proteins on BIV LTR and JDV LTR reporters in BL12 cells. jTat activity is set to 100%. The dotted line in (A) and (E) indicates where the proteins are fused.

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