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Figure 1 | Virology Journal

Figure 1

From: Comparative functional analysis of Jembrana disease virus Tat protein on lentivirus long terminal repeat promoters: evidence for flexibility at its N-terminus

Figure 1

Activation of LTR reporters by wild-type jTat or truncation mutants. (A) Schematic representation of N-terminal (upper) and C-terminal (lower) truncation mutants. Numbers indicate the residue positions. (B) Cells were co-transfected with 25 ng LTR-luc reporter, 50 ng pCMV-LacZ and the indicated amounts of wild-type jTat expression plasmid (pjTat). Total DNA was adjusted to equivalent amounts with pcDNA3.1(-). Luciferase activity was measured 48 h post transfection and normalized to β-galactosidase activity. Asterisk marks the optimal pjTat levels, which are used in subsequent assays. (C) HIV LTR activation in HeLa cells and BIV or JDV LTR activation in BL12 cells by jTat wild-type (WT) or N-terminal truncation mutants. (D) LTR activation by WT or C-terminal truncation mutants. WT activity on each reporter is set to 100% and percentage activation is the relative activity of the indicated mutant. Error bars represent the standard deviation from the mean obtained from more than three independent experiments.

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