Figure 3

Impact of L2 C22A and C28A point mutations upon display of residues 17-36 on the capsid surface. HPV16 pseudovirions containing L2 with wild type or C22S or C28S mutations were bound to the surface of HeLa cells for one hour at 37°C. These cells were either fixed (1 h) or washed and chased at 37°C for five hours prior to fixation (6 h). The exposure of the HPV16 L2 17-36 epitope upon the virion surface was then determined by immunofluorescent staining with the rabbit polyclonal antibody to HPV16 L2 17-36 peptide, as previously described [1, 4].