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Figure 7 | Virology Journal

Figure 7

From: The interaction of hepatitis A virus (HAV) with soluble forms of its cellular receptor 1 (HAVCR1) share the physiological requirements of infectivity in cell culture

Figure 7

Neutralization of wild-type HAV by soluble HAVCR1. HAV.WT-Bsd (5 × 105 TCID50), a wild-type HAV recombinant virus containing a blasticidin selectable marker, or cell culture-adapted HAV (5 × 106TCID50) were incubated with 20 μg of D1 muc-Fc (hatched bars) or PVR-Fc (closed bars) for 2 h at 37°C. Residual infectious wt HAV virus was titrated by the antibiotic resistance titration assay (ARTA) in Huh-7 AI in the presence of 2 μg/ml blasticidin. Residual infectious cell culture-adapted HAV was titrated by an ELISA end point dilution assay [16]. Values are the log10 TCID50 of HAV determined by the Reed and Muench method [30], and the standard deviations are shown as error bars. The neutralization index (NI) of soluble receptor-treated virus compared to mock-treated virus is indicated above each bar. Very significant differences (p < 0.01) in titers of virus treated with D1 muc-Fc or PVR-Fc are indicated with asterisks and their corresponding p-values.

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Virology Journal

ISSN: 1743-422X