Figure 5From: The interaction of hepatitis A virus (HAV) with soluble forms of its cellular receptor 1 (HAVCR1) share the physiological requirements of infectivity in cell cultureEGTA inhibits the alteration of HAV by soluble HAVCR1. Sedimentation analysis of HAV alteration by soluble HAVCR1 in the presence of ethylene glycol bis(beta-aminoethyl ether)-N, N, N', N'-tetraacetic acid (EGTA). Purified HAV virions were incubated with 5 μg of D1 muc-Fc or PVR-Fc for 2 h at 37°C with or without the addition of EGTA and separated by sedimentation in linear 15 to 30% sucrose gradients as described in Figure 2. The HAV antigen in the gradient fractions was detected by ELISA. Data are the mean OD 450 nm from duplicate wells; duplicate values varied by less than 10%. Poliovirus native virions and empty particles labeled with 35S-methionine were used as 160S and 80S sedimentation markers.Back to article page