Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon

Table 1 Primers used for construction of pmSAV3.

Primer	Sequence 5'-3'	REN site
KP1	CCGAATTC GTTAAATCCAAAAGCATACATATATCAATGATGC	EcoRI
KP2	CCCGGGGCGGCCCCAAGGTCGAGAACTGAGTTG
KP3	CCCGGGAGGAGTGACCGACTACTGCGTGAAGAAG
KP4	GGTCTAGA GTATGATGCAGAAAATATTAAGG	XbaI
nsP2SacIF	GAGCTC ATGACTGCGGCTGCC	SacI
nsP3HpaIR	GTTAAC CAAGACTTCCTCTTCGGC	HpaI
AscI3UTRF	GGCGCGCC ATTCCGGTATATAAA	AscI
AscIGFPR	GGCGCGCC TTACTTGTACAGCTCGTCCATGC	AscI
XbaIAgeIKGFPF	TCTAGACCAACC ACCGGTGCCACCATGGTGAGCAAG	XbaI, AgeI
5HHribo2	GGGGAGCTCGCTAGC TGGATTTATCCTGATGAGTCCGTGAGGACG AAACTATAGGAAAGGAATTCCTATAGTCGATAAATCCAAAAGC	SacI, NheI
3HHribo2	CCCGCCGGC GGAGGGGTTAGCTGTGAGATTTTGCATCATTGATATATG TATGCTTTTGGATTTATCGACTATAGGAATTCCTT	NaeI
NotIXbaIPolyAR	CCGCGGCCGCTCTAGA T₂₅ATTGAAAATTTTAAAAACC	NotI, XbaI
NotIXbaIPolyA3R	CCGCGGCCGCTCTAGA T₂₃ATATTGAAAATTTTAAAACC	NotI, XbaI

Restriction enzyme sites that were used during cloning are indicated in the sequence by bold letters.

ISSN: 1743-422X