Figure 1

Cloning and characterization of SAV3 5'- and 3'-ends. The 5'- and 3'-ends of SAV3 were cloned and sequenced from the isolate SAVH20/03 and aligned to the 5'- and 3'-UTRs of other SAVs. A) Alignment of the region in the 3'-UTR containing a sequence homologous to the alphavirus 19 nt CSE (underlined). B) In silico RNA secondary structure analysis using Mfold of SAV3 5'-UTR and partial coding sequence. Red letters indicate coding sequence. C) Predicted RNA secondary structures of the 3'-UTRs of SAV2 and SAV3. Position of the polyadenylated tail is indicated.