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Figure 5 | Virology Journal

Figure 5

From: Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli

Figure 5

Analysis by immunodetection of fractions from asialofetuin affinity chromatography. Five mg of the glycoprotein asialofetuin were immobilized on a 10 ml sepharose column. A total of 25 μg of RTB/p24 purified protein were loaded on to the column. Lane 1, protein not retained by the asialofetuin-sepharose column. Lane 2 column washes. Lane 3 through 5, fractions retained on the column. Immunodetection was performed using the anti-ricin polyclonal antibody at 1/2000 dilution. The arrow indicates the expected protein (57 kDa).

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Virology Journal

ISSN: 1743-422X