Figure 4From: Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coliFunctional assay of RTB/p24 chimeric protein. Capture ELISA was performed using 20 μg of asialofetuin per well. Three concentrations of p24 and RTB/p24 were employed for the assay (1.0 μg, 0.5 μg, 0.25 μg, and 0.125 μg). As primary antibody, monoclonal anti-p24 was used at 1/500 dilution. Samples were done in triplicate and deviations are included.Back to article page