Accumulation of CRL transcripts in CaMV35S-CRL and CRL-RNAi T2 lines. a, RT-PCR showing increased levels of CRL transcripts in CaMV35S-CRL T2-1 and T2-7 plants, contrasting with decreased levels in CRL-RNAi T2-4 and T2-5 as compared with levels from a wild-type plant. b, Expression analysis of CaMV35S-CRL T2-1 and wild-type control by northern blot. c, Small interfering RNA northern blot analysis of CRL-RNAi T2-5 line and wild-type plants. In all cases RNA was isolated from rosettes at 30 days post-germination. For b and c, complete CRL cDNA was used as probe. Ethidium bromide staining of rRNAs is shown as loading control.