Map of plasmid pFGC5941, CRL vectors, CaLCuV A-GFP construct and viral-inoculation method. a, Map of plasmid pFGC5941 indicating 35S promoter, CHSA intron sequence and restriction sites. b, Map of CRL-RNAi construct indicating CRL open read frames cloned in both senses. c, Map of CaMV35S-CRL construct indicating CRL open read frame cloned downstream of 35S promoter. d, Genomic map of CaLCuV A-GFP construct indicating GFP open reading frame and restriction sites. e, Virus inoculation method indicating the inoculated (I) and systemic (S) tissues.