Figure 3

Nedd4 accumulates in the perinuclear region in cells infected with wild-type HSV-2 but not in cells infected with UL56-deficient HSV-2. (A) Images of live cells transfected with a Nedd4-GFP expressing plasmid and sequentially infected with wild-type (186) (top panels) or UL56-deficient (ΔUL56Z) viruses (bottom panels). Nedd4-EGFP accumulated in the perinuclear region after 6 h postinfection (hpi) in cells infected with wild-type viruses, but remained diffuse in cells infected with ΔUL56Z. Scale bars, 10 μm. (B) Western blots of cell lysates infected with wild-type (186) or ΔUL56Z viruses. UL56 was detected after 6 h postinfection in cells infected with wild-type viruses. VP5, a major capsid protein, was detected after 6 h postinfection at equivalent levels in cells infected with wild-type viruses or ΔUL56Z. β-actin was used as a loading control.