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Figure 5 | Virology Journal

Figure 5

From: Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains

Figure 5

RNase H activity of wild-type enzymes and their chimeric derivatives. 5'-32P-labeled 30-mer RNA annealed with its complementary 30-mer DNA strand was incubated at 37°C with the wild-type RTs and their chimeric derivatives under standard reaction conditions. The reactions were analyzed on an 8% denaturing polyacrylamide-urea gel. Panels A and B indicate reactions carried out at lower and higher time points, respectively.

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