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Figure 4 | Virology Journal

Figure 4

From: Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains

Figure 4

Fidelity of DNA synthesis by wild-type enzymes and their chimeric derivatives on 49-mer U5-PBS DNA template in the presence of three dNTPs. The ability of the enzymes to generate and extend mispair in the presence of three dNTPs was assessed on a 49-mer U5-PBS DNA template. The reaction products were analyzed on a denaturing 8% polyacrylamide-urea gel followed by phosphorImager analysis. Lanes 1-4 represent the products formed in the absence of dATP, dCTP, dGTP, and dTTP, respectively. Lane 5 represents the products synthesized in the presence of all four dNTPs. The position of the 17-mer PBS primer is indicated on the left.

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Virology Journal

ISSN: 1743-422X