Figure 1

Immunoblot analysis of yeast lysate from recombinant yeast clones. The proteins were separated by SDS-PAGE on a 12% gel, blotted on to nitrocellulose, reacted with respective antibodies, and detected with colorimetric substrate Fast red and napthol alkaline phosphatase (Fig. 1a). Immunoblot of nucleoprotein (NP) probed with polyhistidine monoclonal antibody. Lane 1, molecular weight marker and lane 2, recombinant yeast cell lysate expressing (NP) (Fig. 1b). Immunoblot of truncated neuraminidase 3 (N3T) and neuraminidase 7 (N7T) probed with c-Myc monoclonal antibody. Lane 1, molecular weight marker; lane 2, recombinant yeast expressing N3T; lane 3, affinity purified N3T; lane 4, recombinant yeast expressing N7T; lane 5, affinity purified N7T. The position of the marker proteins in kD (right) are indicated.