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Table 3 Oligonucleotide primers used for RT-PCR amplification and nucleotide sequencing.

From: The Asia 2 specific signal peptide region and other domains in fusion protein genes characterized Asia 1 and Asia 2 canine distemper viruses

Name

Sequence (5' - 3')a

Nucleotide position

Region

4754F

ACTTGCCCGATCTCAAGCTA

4754-4773

M-F

5211F

AGTGTCTCAAAAGGAGCGAG

5211-5230

F gene

07-5379F

GGACCGACAGTGTCCATTAT

5380-5399

 

5645F

GGCTACAGCTGCACAAATCA

5645-5664

 

6210F

ACTGTCCCGAGGTATGTTGC

6210-6229

 

6751F

TTGGCAGTCTCCTCAGTGTT

6751-6770

 

CDV-HS1

AACTTAGGGCTCAGGTAGTCC

7054-7074

H gene

7661F

GTAGGCAAAGTTTTCCCCCT

7661-7680

 

cdvhf1

TGTGTGTAGAAGAGAGCACTGT

7962-7983

 

8691F

TTTATGACCCAATCCGGACG

8691-8710

 

CDVHS2

ATGCTGGAGATGGTTTAATTCAATCG

8969-8994

H-L

  1. a Forward and reverse primers in italic used for RT-PCR.
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Virology Journal

ISSN: 1743-422X