Skip to main content
Figure 4 | Virology Journal

Figure 4

From: Stable replication of the EBNA1/OriP-mediated baculovirus vector and its application to anti-HCV gene therapy

Figure 4

Detection of EBNA1 protein and siRNA in Ac-EP-shRNA452 infected cells. A Western blot analysis of EBNA1 expression in baculovirus-infected Huh7 cells. Cell lysates were prepared 7 days and 14 days post-infection from cells infected with different viruses. Lane 1: Ac-sh452; lane 2: Ac-EP-sh452; lane 3: Ac-EP-control-shRNA. B Expression of siRNA by a baculovirus vector. To demonstrate the intracellular expression of the shRNA construct in the respective siRNA, Huh-7 cells were infected with Ac-EP-shRNA452. The mixture was run on a 15% polyacrylamide TBE urea gel after 3, 7, and 14 days.

Back to article page