Table 1 Summary of properties of identified HIV-1 resistant cellular genes
Target genes | Main known function | Cellular localization | C'# | Perturbation | siRNA validation |
|---|---|---|---|---|---|
CASD1 CAS1 domain-containing protein 1 precursor | O-acetyl-transferase | Multi-pass membrane protein | 7 | Antisense intron 4 Down-regulation | Yes |
HECW2 HECT, C2 and WW domain-containing protein 2 | NEDD4-like E3 ubiquitin-protein ligase 2 | Intracellular | 2 | Sense intron15 ATG(sc) exon 7 Down-regulation (DN) | Yes |
ROBO1 roundabout protein homolog 1 | axon guidance receptor and cell adhesion receptor | Type I membrane protein | 3 | Sense intron 3 ATG(sc) exon 3 Down-regulation (DN) | Yes |
NLGN1 member of the neuroligin family | splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses | Neuronal cell surface | 3 | Antisense intron 3 Down-regulation | Yes |
DZIP3 (DAZ-interacting protein 3) | E3 Ubiquitin ligase proteins. Specifically bind RNA. Also called huRUL138 (RNA-binding ubiquitin ligase of 138 kDa) | Intracellular | 3 | Antisense intron 1 Down-regulation | Yes |
CAMSAP1L1 calmodulin regulated spectrin-associated protein | may be involved in spectrin's function as a cytoskeletal protein providing a scaffolding and maintenance of plasma membrane | Membrane | 1 | Antisense intron 2 Down-regulation | Yes |
GSTCD glutathione S-transferase (GST), C-terminal domain | structural domain of GST, which conjugates reduced glutathione to a variety of targets to facilitate detoxification of the targets | Intracellular | 4 | Antisense intron 5 Down-regulation | Yes |
CPSF1 Cleavage and polyadenylation specificity factor | It recognizes the AAUAAA signal in the pre-mRNA and facilitates both RNA cleavage and polyA synthesis | Nucleus | 8 | Antisense intron 2 Down-regulation | Yes |
GDAP2 ganglioside induced differentiation associated protein 2 | a signal transduction pathway during neuronal development | Intracellular | 1 | Sense last exon Down-regulation (DN) | Yes |
TNRC6A trinucleotide repeat containing 6 protein | post-transcriptional gene silencing through the RNAi and microRNA pathways. | Cytoplasmic bodies | 16 | Sense intron 4 ATG(sc) exon 1 Down-regulation (DN) | Yes |
TTC21B tetratricopeptide repeat domain 21B | may negatively modulate SHH (Sonic Hedgehog) signal transduction and may play a role in retrograde intraflagellar transport in cilia | Intracellular | 2 | Antisense intron 4 Down-regulation | Yes |
ATP8A1 aminophospholipid transporter (APLT), Class I, type 8A, member 1 | transport of aminophospholipids from the outer to the inner leaflet of various membranes and the maintenance of asymmetric distribution of phospholipids, mainly in secretory vesicles | Membrane | 4 | Sense intron 4 ATG(sc) exon 1 Down-regulation (DN) | Yes |
Unknown ESTs | BE066906, AW817767, EB388641 | unknown | 3 | Sense | n.d. |
| Â | AW300614 | unknown | 2 | Antisense | n.d. |