Figure 8
Validation of candidate genes providing HIV-1 resistance via siRNA targeting of naïve T lymphocytes. Naïve T cells were challenged one day after electroporation with the siRNAs specific for the human targets listed in Table 1. Production of the progeny virus 48 h post-infection were quantified using TZM-bl cells. Percentages of virus production from each siRNA treatment against the non-targeting siRNA control sample are shown. Note that siRNA against Tat and Rab6A provided positive controls for established HIV-1 siRNA targets. (A) Data from siRNA transfected MT4 cells followed by X4 tropic HIV-1NL4-3 infection. Percentages of viable cells after siRNA transfection and viral infection (2 dpi) are shown in the bottom. (B) Results from siRNA transfected PM1 cells followed by infection with the R5 tropic HIV-1Mei virus.