The viral amplicon construct, VA/pCAMBIA1391Z, replicates in both dicot and monocot plants. The plants (Tomato, Tobacco, Arabidopsis and Rice) were agro-infiltrated with this construct, and the release of episome was checked by PCR using internal primers. The amplification products (1.4 kb) were separated on 1% agarose gel and are presented in panels A, B, C and D for Tomato, Tobacco, Arabidopsis and Rice respectively. The band intensities were quantified and the normalized values with respect to corresponding loading controls (ACTIN) are plotted as bar graphs in panels E, F, G and H respectively. In these panels the respective values at 3, 1, 4 and 4 dpi were arbitrarily assigned as 100%. The standard deviations shown are based on three independent experiments.