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Table 2 Clinical samples used to evaluated the performance of the OPV/PPV nested-multiplex PCR

From: Nested-multiplex PCR detection of Orthopoxvirus and Parapoxvirus directly from exanthematic clinical samples

State/Year

 

N° of specimens

Sourcea

Designation

Specimen

Positive samples

Result

Reference

Minas Gerais, 2005

 

2

B

GP1V, GP2V

scab

2

OPV

Trindade et al., 2006

Minas Gerais, 2005

 

11

B/H

SV

scab and vesicle

10

OPV

Trindade et al., 2007

Minas Gerais, 2003

 

1

B

PSTV

scab

1

OPV

Leite et al., 2005

Minas Gerais, 2005

 

13

B/H

MARV

scab and vesicle

11

OPV

Abrahão et al., upubl. Data

Espírito Santos, 2008

 

4

B/H

LINV

scab and vesicle

5

OPV

Abrahão et al., upubl. Data

Minas Gerais, 2005

 

5

B/H

RPLV

scab and vesicle

5

OPV

Abrahão et al., upubl. Data

Minas Gerais, 2005

 

8

B/H

JQRV

scab and vesicle

7

OPV

Abrahão et al., upubl. Data

Minas Gerais, 2008

 

8

B

PRGV

scab

8

OPV

Abrahão et al., upubl. Data

Minas Gerais, 2008

 

4

H

ARGV

vesicle

4

OPV

Abrahão et al., upubl. Data

Minas Gerais, 1990

 

1

C

ORF-A

sacb

1

PPV

Mazur & Machado, 1990

Pernambuco, 1993

 

2

C

NE1, NE2

scab

2

PPV

Mazur et al., 2000

Mato Grosso, 2005

 

5

O

MT05

scab

5

PPV

Abrahão et al., 2009

 

Total

64

Positivity 61 (95,31%)

  1. aB = bovine; H = human; C = caprine; O = ovine