Table 1 Selected primers for the OPV/PPV nested-multiplex PCR
Genus | Target gene | Primer sequence (5' - 3') | Reference | |
|---|---|---|---|---|
OPV | vgf | 1st step | vgfF: CGCTGCTATGATAATCAGATCATT | Fonseca et al., 1998 |
vgfR: GATATGGTTGTGCCATAATTTTTAT | ||||
Nested step | vgfF2: ACACGGTGACTGTATCCA | This study | ||
vgfR2: CTAATACAAGCATAATAC | ||||
PPV | b2l | 1st step | OVB2LF1: TCCCTGAAGCCCTATTATTTTTGT | Hosamani et al., 2006 |
OVB2LR1: GCTTGCGGGCGTTCGGACCTTC | ||||
Nested step | PPP-1: GTCGTCCACGATGAGCAG | Inoshima et al., 2000 | ||
PPP-4: TACGTGGGAAGCGCCTCGCT |