Figure 5

Poly-caspase inhibitor alters the pathway of MNV-1 infection. RAW 264.7 cells were infected with MNV-1 in the presence of the poly-caspase inhibitor, Boc-D-FMK (black bars), and without the inhibitor (striped bars). Trypan blue staining shows that cell viability decreases much more in the presence of inhibitor (A). Only cells infected in the absence of the caspase inhibitor show an increase in chromatin condensation as monitored with Hoechst stain (B). Cells infected in the presence of inhibitor lose membrane integrity more rapidly as monitored by propidium iodide (PI) staining (C). (n = 3 for all panels).