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Figure 9 | Virology Journal

Figure 9

From: Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo

Figure 9

In-vivo effect of the ORF7b deletion. Golden Syrian hamsters were infected with 104 PFU of rSCV and r7bΔTMD (x-axis). Heat inactivated rSCV served as mock control. For each point of time post infection, three animals per virus variant were sacrificed (animals 1, 2, 3 as identified on the x-axis). Lungs were taken in total. Viral titers were determined by plaque assay and viral RNA was quantified by real-time RT-PCR. Light grey bars represent log copies of viral RNA, dark grey bars represent PFU per g lung tissue. The arrow indicates one animal with failure of virus replication.

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Virology Journal

ISSN: 1743-422X