Table 1 Primers used for fragments amplification and sequencing of complete genome of HBV genotype D
Fragment no. (bp) | Primers* | Polarity | Sequence (5'→'3) | Domain | Position |
|---|---|---|---|---|---|
1 (379) | HBPr1 | Forward | GGGTCACCATATTCTTGGG | HBPol | 2850–2860 |
|  | HBPr2 | Forward | GAACAAGAGCTACAGCATGGG | HBPol/PreS1 | 2867–2888 |
|  | HBPr3 | Reverse | CCACTGCATGGCCTGAGGATG | PreS1/PreS2/HBPol | 3226–3246 |
2 (891) | HBPr14 | Forward | TGGGGTGGAGCCCTCAG | PreS1/HBPol | 3104–3120 |
|  | HBPr94 | Reverse | GGTAWAAAGGGACTCAMGATG | HBPol/HBsAg | 775–795 |
|  | HBPr135 | Reverse | CARAGACAAAAGAAAATTGG | HBPol/HBsAg | 803–822 |
3 (541) | HBPr440 | Forward | TATGGATGATGTGGTATTGGG | HBPol/HBsAg | 738–758 |
|  | HBPr113 | Reverse | CCGGCAGATGAGAAGGCACAGACGG | HBX/HBPol | 1549–1574 |
|  | HBPr374 | Reverse | GTTCCGCAGTATGGATCGGCAGAGG | HBPol | 1255–1279 |
4 (319) | HBPr110 | Forward | CCTCTGCCGATCCATACTGCGGAAC | HBPol | 1255–1279 |
|  | HBPr113 | Reverse | CCGGCAGATGAGAAGGCACAGACGG | HBX/HBPol | 1549–1574 |
5 (560) | HB1 | Forward | GCCAAGTGTTTGCTGACGC | HBPol | 1174–1192 |
|  | HB2 | Forward | CCATACTGCGGAACTCCTAG | HBPol | 1265–1284 |
|  | HB3 | Reverse | AAAGTTGCATGGTGCTGGTG | HBX | 1803–1822 |
6 (726) | HBPr86 | Forward | ACATAAGAGGACTCTTGGAC | HBX | 1652–1671 |
|  | HBPr87 | Forward | TACTTCAAAGACTGTGTGTTTA | HBX | 1704–1723 |
|  | HBPr111 | Reverse | CTGCGAGGCGAGGGAGTTCTTCTTC | Core/HBPol | 2406–2430 |
7 (585) | HBPr33 | Reverse | CTGAGGGCTCCACCCCA | PreS1/HBPol | 3104–3120 |
|  | HBPr446 | Forward | GGAGTGTGGATTCGCACTCC | Core | 2303–2323 |
|  | HBPr448 | Reverse | CCCATGCTGTAGCTCTTGTTC | HBPol/PreS1 | 2868–2888 |