Figure 2

Isolation of raft membranes from HSB-2 cells infected with HHV-6A; the detection of viral proteins. Bottom-loaded sucrose step gradients (fraction 1 represents the top of the gradient) were analyzed by immunoblotting. (A) Immunoblots of proteins from each fraction (equal volume loaded) were labeled with anti-gH (a), gL (b), gQ1-80K(c), gQ1-74K(c), gQ2-37K(d), gQ2-34K(d), gB-112K (e), gB-60K(e), gO-120K(f), gO-80K (f), or IE1(g) antibody for HHV-6A proteins. GM1 (h), which migrated with the dye front, was detected by reaction with HRP-coupled cholera toxin. Same photo used in Fig. 1B (f) was used for GM1. The positions of the viral proteins are indicated on the right of the figure. P indicates pellet. The sample loaded here was same one used in Fig. 1B. The experiment was done three times independently, and one of three experiments was shown here. All of these blots came from the same experiment, but the exposure time of each blot was not identical. (B) The blots were quantitated by densitometry analysis by KODAK MI software and the amounts in DRM or soluble fractions were determined as a percentage of the total of all the blots. Number indicates each percentage. DRM; detergent resistant membrane.