Replication kinetics and histopathology of the RGMal/01 in inoculated ferrets. Three ferrets were inoculated i.n. with 5 × 105TCID50 of RGMal/01 virus. At 8, 24, and 68 hpi, each ferret was euthanized. (A) Brain, olfactory bulb (OB), nasal turbinate (NT), trachea, lung, kidney, liver, spleen and intestine were harvested, weighted and homogenized in PBS. Virus in these tissues was titrated in MDCK cells. The titers are expressed as log10 numbers of TCID50 per ml of 10% (w/v) tissue homogenate. The detection limit is 0.699 log10TCID50/ml. (B) Lung was collected and fixed with formalin. The lung of a mock-infected ferret was collected as negative control. Sections of 5 μm thick were cut and routinely processed for H&E staining. Note that the severe inflammatory infiltration is progressing in the infected lung.