Figure 2From: Development of a RVFV ELISA that can distinguish infected from vaccinated animalsTitration of antigens with various sera. Antigens were serially diluted and coated onto EIA plates. After overnight binding and then blocking, the plates were incubated with a 1:100 dilution of human (A), rat (B) or goat (C) sera and the appropriate secondary antibody as described in materials and methods. P is a positive control serum, N is a negative control serum and S is a secondary alone control.Back to article page