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Figure 1 | Virology Journal

Figure 1

From: Development of a RVFV ELISA that can distinguish infected from vaccinated animals

Figure 1

Expression and purification of the RVFV N and NSs antigens. Protein samples were mixed with reducing sample buffer and run on SDS PAGE gels as described in Methods. (A) Induction and purification of N and NSs. M: molecular weight maker. 1: uninduced whole cell lysate from E. coli that was transformed with a plasmid that expressed either the RVFV N or NSs protein, Lane 2: whole cell lysate from the samples after induction with IPTG, Lane 3: purified N or NSs protein. Gels were transferred to PVDF membranes and western blotted for either the NSs protein (1B) or the N protein (1C) with human polyclonal or mouse monoclonal sera respectively. On each gel lanes 1, 2, and 3 represent uninduced whole cell lysate, induced whole cell lysate and purified protein.

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