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Table 2 Effects of inhibitors on kinetics parameters of A/PR/8/34 (H1N1) sialidase activity

From: Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities

Kinetic parameter

No inhibitor

OC

(4 nM)

PVP-I

(75 μg/ml)

DANA

(5 μM)

Vmax(μmol/l.min)

1.70

1.68

1.22

1.69

Km (μmol/l)

14.66

103.20

77.72

184.10

Km (μg/ml)a

7.17

-

-

-

Vmax/Km(1/min)

0.12

0.02

0.02

0.01

Type of inhibition

-

Competitive

Mixed

Competitive

Ki(nM)

-

0.66

-

432.60

Ki (μg/ml)

-

-

11.74

-

Kis (μg/ml)

-

-

190.63

-

Km/Ki

-

2.21 × 104

0.61

33.90

Km/Kis

-

-

0.04

-

  1. aMW of MUNA is 489.39.
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Virology Journal

ISSN: 1743-422X