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Table 2 Effects of inhibitors on kinetics parameters of A/PR/8/34 (H1N1) sialidase activity

From: Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities

Kinetic parameter No inhibitor OC
(4 nM)
PVP-I
(75 μg/ml)
DANA
(5 μM)
Vmax(μmol/l.min) 1.70 1.68 1.22 1.69
Km (μmol/l) 14.66 103.20 77.72 184.10
Km (μg/ml)a 7.17 - - -
Vmax/Km(1/min) 0.12 0.02 0.02 0.01
Type of inhibition - Competitive Mixed Competitive
Ki(nM) - 0.66 - 432.60
Ki (μg/ml) - - 11.74 -
Kis (μg/ml) - - 190.63 -
Km/Ki - 2.21 × 104 0.61 33.90
Km/Kis - - 0.04 -
  1. aMW of MUNA is 489.39.