Figure 6From: Analysis of a conserved RGE/RGD motif in HCV E2 in mediating entryConformation of HCV E2 glycoprotein substitutions within conserved RGE/RGD motif. 293 T cells transfected with HCV E1E2 wt or RGE/RGD mutant expression vectors were lysed 24 h post-transfection. Cleared cell lysate was incubated with AR3A (C1) conformational antibody to assess conformation of mutations. Immunoprecipitated proteins were detected by subsequent Western Blot analysis of E2. HCV E2 R614A was used as a negative control.Back to article page