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Figure 5 | Virology Journal

Figure 5

From: Analysis of a conserved RGE/RGD motif in HCV E2 in mediating entry

Figure 5

Binding of hepatitis C virus E2 glycoproteins containing RGE substitutions to soluble CD81. 293 T cells transfected with HCV E1E2 wt of mutant expression vectors were lysed 24 h post-transfection. Cleared cell lysate was incubated with soluble CD81-GST fusion protein. Binding to CD81 was determined by Western blot analysis of E2 and the GST tag. As a negative control, GST protein without soluble CD81 was incubated with HCV wt.

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