Figure 4

Blocking RGE/RGD sites on Huh7 cells prior to hepatitis C virus pseudoparticle challenge. (A) Huh7 cells were incubated at 4°C for 1 h with ECM proteins then washed and infected with HCVpp. (B) Inhibition of HCVpp infectivity by peptides GRGDTP, GRGESP, RGD-E2 or RGE-E2 was assessed by incubating Huh7 cells with increasing amounts of peptide at 4°C for 1 h, washing and infecting with HCVpp. Infecitivity for both was determined 72 h PI using a luciferase reporter assay.