Figure 3
From: Analysis of a conserved RGE/RGD motif in HCV E2 in mediating entry
Expression and incorporation of hepatitis C virus E1E2 glycoproteins in producer cell lysate and HCVpp. (A) 293 T producer cells were lysed on analyzed by Western blot analysis using anti (α)-E2. Detection of actin was performed as a loading control. (B) Incorporation of HCV glycoproteins into HCVpp was determined by pelleting the virus through a 20% sucrose cushion followed by Western blot analysis. Detection of p24 capsid protein was performed as a loading control.