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Table 6 Primers utilized in RT-PCR

From: Sequence diversity on four ORFs of citrus tristeza virus correlates with pathogenicity

Primer

Name

Genomic region

Nucleotide sequence (5' → 3')

Primer localization (nt)a

Size of product (pb)

IRA 9

P349-Bc

5'TTGGTTGGTGGTGAGTCTGC3'

1555–1574

876

IRA 10

 

5'GTGCCACTCGGAAAACTGAAAT3'

2414–2435

 

IRA 11

P349- Cc

5'TGAGCAGATCGGAGGTCTTG3'

6921–6940

1050

IRA 12

 

5'ACGTCATCGTCCAAATCCA3'

7952–7970

 

IRA 1

CP

5'ATGGACGACGAAACAAAGAAATTG3'

16116–16139

672

IRA 2

 

5'GC TCAACGTGTGTTAA3'

16774–16787

 

IRA 5

P13

5'GACTTAGACACGAAGTGACC3'

17250–17269

360

IRA 6

 

5'CTAAAGTAAGCTCGCATATTG3'

17721–17741

 

IRA 7

P23

5'CGTGTAGGTTAATACGCTTCTC3'

18267–18288

630

IRA 8

 

5'CTTATTCCGTCCACTTCAATCAG3'

18982–19004

 
  1. a numbering according to full-length of CTV isolate T385.
  2. b + forward primer; - reverse primer
  3. c B and C are the regions of the ORF p349 that were analyzed in this job.