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Figure 1 | Virology Journal

Figure 1

From: 9-aminoacridine Inhibition of HIV-1 Tat Dependent Transcription

Figure 1

9AA inhibits HIV-LTR transcription. A) CEM cells were transfected with 2.5 μg HIV-LTR CAT and 0.5 μg of pc-Tat by electroporation. Twenty-four hours post-transfection, cells were treated with DMSO, 9AA (0.1, 0.5, 1, or 2.5 μM), or 100 nM Flavo (flavopiridol). Cells were harvested 48 hours post transfection and processed for CAT assays. CAT assays were performed with 4 mM acetyl CoA, 5 μl of 14C-chloramphenicol (40 mCi/mmole), 10 μl of protein extracts, and 18 μl of water. Reactions were carried out at 37°C for 30 minutes. Samples were extracted with ethyl acetate, dried, and separated by TLC. B) TZM-bl cells were transfected with 1.0 μg of Tat and treated the next day with DMSO, 9AA (0.1, 0.5, 1, or 2.5 μM), or 100 nM flavopiridol. Cells were processed 48 hours post drug treatment for luciferase assays. Assays were performed in triplicate and an average value is shown plus standard deviation. C) Structures of 9AA and derivatives. D) TZM-bl cells were transfected with 1.0 μg of Tat and treated the next day with DMSO, 1 or 10 μM of 9AA, 2AA, 4AA, AH, 4AQ, or 100 nM flavopiridol. Cells were processed 48 hours post drug treatment for luciferase assays. Assays were performed in duplicate and an average value is shown.

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