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Table 2 Primers.

From: Diversification of West Nile virus in a subtropical region

  Forward Primer Reverse Primer
Envelope 1st
(1042–1857)
5'-GAAGGCGATAGTTGTGTGACCA-3'
(1042–1063)
5'-TGTTCCCTTCAGCTGCAACTT-3'
(1834–1854)
Envelope 2nd
(1632–2459)
5'-CCTTGGAGCAGTGCTGGAAGTA-3'
(1636–1657)
5'-TTCACGGAGAGGAAGAGCAGAA-3'
(2438–2459)
NS3 1st
(5085–5908)
5'-CGGCTCATACATAAGCGCGAT-3'
(5085–5105)
5'-TTGGTTTCACACTCTTCCGGC-3'
(5888–5908)
NS3 2nd
(5514–6318)
5'-TTCCACAAAGGTCGAGCTAGG-3'
(5514–5534)
5'-CCTAGGACCATCAAAGCACCA-3'
(6298–6318)
NS3 3rd
(5950–6726)
5'-CCATCTGCAGTGACAGCAGCTA-3'
(5950–5971)
5'-TTCGTTCCTGGAACTTCAGCC-3'
(6756–6776)
  1. Primer sequences used to sequence the envelope and NS3 regions of the samples.
  2. *NT positions refer to amplicon location with respect to "WNV RNA, Complete Genome" GenBank accession number M12294