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Table 4 Primers used in reverse transcription and amplification of portions of the S and M segments of Sin Nombre virus genotypes

From: Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana

PCR step

SNV segment

Primer name

Sequence

RT

S

SNV-S5'T

TAGTAGTAGACTTCKTRAAGAGCTACT

1° reaction

S

SNV-S41s

GGAATGAGCACCCTCAAAGAAGTGCAAGACAAC

1° reaction

S

PSE-S1064r

ATRGTRTTYCTCATATCCTG

2° reaction

S

SNV-S143s

TGGACCCMGATGAYGTTAACAA

2° reaction

S

SNV-S1000r

GACAYCGATCWGGNGCACATGCAAARACCC

RT

M

SNV-M2730s

CTTTTAGAAAGAWMTGTGSRTTTGC

1° reaction

M

SNV-M2730s

CTTTTAGAAAGAWMTGTGSRTTTGC

1° reaction

M

SNV-M3023r

CCTACTCCTGAACCCCAGGCCCCG

2° reaction

M

SNV-M2764s

CCAACATGTGAGTATCAAGGCAACACAGTGCTGG

2° reaction

M

SNV-M2966r

GGKKTWTCACTTAGRTCYTGRAAGG

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Virology Journal

ISSN: 1743-422X