Capsid-incorporated antigens elicit a varied T cell response. (A-B) C57BL/6J mice were immunized with 1010VP of Ad vectors. On day 40, these mice were intravenously boosted with the same dose of the same vectors. A single-cell suspension of spleen cells was prepared on day 9 after secondary virus infection. Cells were stained with a fluorescent labeled anti-CD4 antibody and then permeabilized in intracellular stain with fluorescent conjugated antibodies against IL-4 or IFN-γ. Samples were acquired on a FACSCalibur and data were analyzed with FlowJo software. Values are expressed as the mean ± standard error of three replicates.